Yakakomba acute kufema syndrome inozivikanwa seCOVID-19 chirwere - nekuda kweSARS-CoV-2 hutachiona - inozivikanwa kupararira kuburikidza nemadonhwe ekufema uye nepedyo.Mutoro weCCIDID-19 wakanyanya kuomarara muLombardy nePo Valley (Northern Italy), [2] inzvimbo inoratidzwa nekuwanda kwakawanda kwezvinhu, izvo zvinotozivikanwa kuti zvinokonzeresa hutano hwevanhu.[3]Huwandu hwematunhu huripo hweItari pamusi waKubvumbi 12 hunoratidza kuti vangangosvika makumi matatu muzana yevanhu vane hunhu vachiri kugara muLombardy (vanenge makumi mana muzana kana tichifunga nezvenyaya dzese dzakasimbiswa kubva pakutanga kwedenda), ichiteverwa naEmilia Romagna (13.5%). , Piedmont (10.5%), neVeneto (10%).[2]Aya matunhu mana ePo Valley anoita 80% yevakafa vese vakarekodhwa muItari uye 65% yeIntensive Care Units yekubvuma. [2]
Tsvagiridzo yakaitwa neHarvard Chikoro chePublic Health inoita senge ichisimbisa hukama pakati pekuwedzera kwehuwandu hwePM uye huwandu hwevanhu vanofa nekuda kweCCIDID-19 muUS[4] Mukutaurirana kwakapfuura, takafungidzira mukana wekuti SARS-CoV-2. hutachiona hunogona kunge huripo pane chimwe chinhu (PM) panguva yekupararira kwehutachiona, [5,6] nguva dzose nehumbowo hwagara huripo.
inowanikwa kune mamwe mavhairasi.[7-15] Zvisineyi, nyaya yemhepo yakabatana nePM-yakabatana microbiome, kunyanya munzvimbo dzemumaguta, inoramba isingatarisirwi,[16] uye - parizvino - hapana munhu achiri kuita zvidzidzo zvekuyedza zvakanangwa. pakusimbisa kana kusasanganisa kuvapo kweSARS-CoV-2 paPM.
Pano, tinopa mhedzisiro yekutanga yeongororo yatakaita pa34 PM10 samples dzekunze / nendege PM10 kubva kune maindasitiri saiti yeBergamo Province, yakaunganidzwa nemasamplers maviri emhepo akasiyana mukati menguva inoenderera ye3-mavhiki, kubva Kukadzi 21st kusvika Kurume. 13th.
Kutevera nzira inotsanangurwa naPan et al.muna 2019 (yekuunganidza, particle sizeng uye kuonekwa kwehutachiona hwemhepo), [17] PM samples dzakaunganidzwa pamafirita equartz nekushandisa lowvolume gravimetric air sampler (38.3 l/min for 23 h), inoenderana nereferenzi nzira EN12341 :2014 yePM10 yekutarisa.Nyaya yakavharirwa pamafirita ne99.9% yakafananakuchengetwa kweaerosol, kuchengetwa nemazvo uye kuendeswa murabhoritari yeApplied uye Comparative Genomics yeTrieste University.Tichifunga nezve "zvakatipoteredza" chimiro chemuenzaniso, unofungidzirwa kuti wakapfuma mune inhibitors yeDNA polymerases, takaenderera mberi nekubviswa kweRNA nekushandisa Quick RNA fecal ivhu microbe kit yakagadziridzwa kune rudzi rwemasefa.[18]Half sefa yakakungurutswa, rutivi rwepamusoro rwakatarisa mukati;mune 5 ml polypropylene chubhu, pamwe chete nemabheji akapihwa mukiti.Kubva pakutanga 1 ml ye lysisbuffer, takakwanisa kuwana inenge 400 ul yemhinduro, iyo yakabva yagadziriswa sezvinotsanangurwa nemaitiro emitemo, zvichiita kuti pave nekuguma kwekupedzisira kwe15 ul.Mushure mezvo, 5 ul yakashandiswa kuongororwa kweSARS-CoV-2.Tichifunga nezvekwakabva sampuli, qScript XLT 1-Nhanho RT-qPCR ToughMix yakashandiswa.[19]Iyo amplification masisitimu aive ayo eprotocol yakagadzirwa naCorman et al, yakabudiswa pawebhusaiti yeWHO [20].
Muedzo uyu waive wakanangana nekusimbisa kana kusasanganisa huvepo hweSARS-CoV-2 RNA pachinhu chaicho.Ongororo yekutanga yakashandisa "E gene" semucherechedzo wemamorekuru uye yakaburitsa mhedzisiro yakanaka pagumi nemashanu kubva pagumi nematanhatu mafirita kunyangwe, sezvataigona kutarisira, iyo Ct yaive pakati pe36-38 kutenderera.
Mushure meizvozvo, takadzokorora ongororo ye6 yemafirita akanaka (atova akanaka ku "E gene") nekushandisa "RtDR gene" semucherechedzo wemamorekuru - iyo yakanangana neSARS-CoV-2 - inosvika mashanu akakosha mhedzisiro. ye positivity;kudzora miedzo yekubvisa positivity yenhema yakaitwawo zvakabudirira (Fig. 1).
Kudzivirira kupera kwezvakashomeka zvesampling zvinhu zviripo, mamwe maRNA akasara akaendeswa kuchipatara cheYunivhesiti Hospital (imwe yenzvimbo dzekiriniki dzakabvumidzwa neHurumende yeItaly yeSARS-CoV-2 bvunzo dzekuongorora), kuitira kuti iite yechipiri. parallel blind test.Iyi yechipiri murabhoritari yekiriniki yakaedza 34 RNA zvinyorwa zveE, N uye RdRP majini, ichishuma 7 mhedzisiro yakanaka yeinenge imwe yeatatu marker genes, ine positivity yakasimbiswa zvakasiyana kune ese matatu mamakisi (Fig. 2).Nekuda kwechimiro chesample, uye tichifunga kuti sampling haina kuitwa nekuda kwekuongororwa kwekiriniki asi yekuongorora kusvibiswa kwezvakatipoteredza (tichifunga zvakare kuti mafirita akachengetwa kweinenge mavhiki mana asati aitwa mamolecular genetic analysis, se.mhedzisiro yekuvharwa kweItaly), tinogona kuratidza kuti takaratidza zvine musoro kuvepo kweSARS-CoV-2 viral RNA nekuona yakanyanya kujeka "RtDR gene" pamasefa masere.Nekudaro, nekuda kwekushaikwa kwezvinhu zvekuwedzera kubva kumasefa, hatina kukwanisa kudzokorora nhamba yakakwana yebvunzo kuratidza positivity kune ese matatu mamorekuru mamaki panguva imwe chete.
Uhwu ndihwo humbowo hwekutanga hwekuti SARS-CoV-2 RNA inogona kunge iripo pazvinhu zvekunze, nekudaro zvichiratidza kuti, mumamiriro ekugadzikana kwemuchadenga uye kuwanda kwakanyanya kwePM, SARS-CoV-2 inogona kugadzira masumbu ane kunze PM uye - ne kuderedza kupararira kwavo coefficient - kuwedzera kuenderera kwehutachiona mumhepo.Zvimwe zvinosimbisa zvekutanga izvihumbowo huri kuenderera mberi, uye hunofanirwa kusanganisira ongororo yenguva-chaiyo nezve kukosha kweSARS-CoV-2 pamwe nehutsinye hwayo kana yakashambadzirwa pazvinhu zvidiki.Parizvino, hapana fungidziro inogona kuitwa nezve kuwirirana pakati pekuvapo kwehutachiona paPM uye COVID-19 kubuda.Dzimwe nyaya dzinofanirwa kutariswa zvakanyanya ndeyeavhareji yekutariswa kwePM pakupedzisirainodiwa kune inogona "kusimudzira maitiro" ekutapukira (kana zvichinge zvasimbiswa kuti PM anogona kuita se "mutakuri" weiyo hutachiona hwedonhwe nuclei), kana kunyangwe mukana weiyo theoretic wekubaiwa nekuda kwekuratidzwa kushoma kwedosi pazasi pezvikumbaridzo zvePM. .
Fig.1 Amplification curves yeE (A) uye RdRP genes (B): mitsetse yakasvibirira inomiririra mafiritsi akaedzwa;cross linesinomiririra zvinyorwa zvemareferensi;mitsetse mitsvuku inomiririra kukwidziridzwa kwemasamples akanaka.
Fig.2.Mhedzisiro yakanaka (yakaiswa X) yeE, N uye RdRP genes inowanikwa kune ese makumi matatu neshanu PM10 samples.mafirita akaedzwa muongororo yepiri yakafanana.
Leonardo Setti1, Fabrizio Passarini2, Gianluigi De Gennaro3, Pierluigi Barbieri4, Maria Grazia Perrone5, Massimo Borelli6, Jolanda Palmisani3, Alessia Di Gilio3, Valentina Torboli6, Alberto Pallavicini6, Maurizio Ruscio7, Priless Piscirotelli8, Alessia Di Gilio8,
1. Dept. Industrial Chemistry, University of Bologna, Viale del Risorgimento – 4, I-40136, Bologna, Italy
e-mail: leonardo.setti@unibo.it
2. Interdepartmental Center for Industrial Research "Renewable Sources, Environment, Blue Growth, Energy",
University of Bologna, Rimini, Italy e-mail: fabrizio.passarini@unibo.it
3. Dhipatimendi reBiology, Yunivhesiti "Aldo Moro" yeBari, Bari, Italy
e-mail: gianluigi.degennaro@uniba.it; alessia.digilio@uniba.it; jolanda.palmisani@uniba.it
4. Dhipatimendi reMakemikari uye Pharmaceutical Sayenzi, Yunivhesiti yeTrieste, Trieste, Italy.
e-mail: barbierp@units.it
5. Environmental Research Division, TCR TECORA, Milan, Italy
e-mail: mariagrazia.perrone@tcrtecora.com
6. Dhipatimendi reHupenyu Sayenzi - University of Trieste, Trieste, Italy
e-mail: borelli@units.it; torboli@units.it; pallavic@units.it
7. Division of Laboratory Medicine, University Hospital Giuliano Isontina (ASU GI), Trieste, Italy
email: maurizio.ruscio@asugi.sanita.fvg.it
8. Italian Society of Environmental Medicine (SIMA), Milan, Italy
e-mail: priscofreedom@hotmail.com; alessandro.miani@unimi.it
9. Dhipatimendi reScience Environmental uye Poicy, University of Milan, Milan, Italy
e-mail: priscofreedom@hotmail.com; alessandro.miani@unimi.it
Munyori anowirirana:
Leonardo Setti, Department of Industrial Chemistry, University of Bologna Viale del Risorgimento 4, 40136, Bologna, Italy; e-mail: leonardo.setti@unibo.it
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Kuonekwa kweiyo 2019 novel coronavirus (2019-nCoV) neiyo chaiyo-nguva RT-PCR.Eurosurveillance, 25(3), inowanikwa pa:.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988269/
Pakutanga: https://doi.org/10.1101/2020.04.15.20065995
Nguva yekutumira: Kubvumbi-18-2020