Gravis syndrome respiratoriae acutae quae morbus COVID-19 - propter virus SARS-CoV-2 - per stillicidium respiratorii et contactum arctum divulgare dignoscitur.Gravissimum erat onus COVID-19 in Lombardia et in Padi valle (Italiam Septentrionalem), [2] area propria intentionibus materiarum particularium, quae iam notae sunt effectibus negativis ad salutem hominum producendam.Figurae regionales praesto pro Italia die 12 mensis Aprilis ostendunt circiter 30% hominum actuosorum in Lombardia adhuc vivere (circiter 40% si considerantes casus altiores ab initio pestilentiae confirmatas), secuti sunt Aemiliam Romandiolam (13.5%). , Pedemontanus (10,5%), et Venetus (10%).Hae quattuor regiones Padi Vallis rationem 80% totalium defunctorum in Italia conscriptas et 65% intensivae Curae Unitates concessionum.
Investigatio facta a Scholae Publicae Harvardianae confirmari videtur consociationem inter incrementa in AM concentratione et mortalitatis rates ob COVID-19 in US [4] Prioribus communicationibus possibilitatem quam SARS-CoV-2 hypothesizavimus. Virus in materia particulari (PM) per dilatationem contagionis [5,6] praesens esse potuit, cum argumentis iam constant
Attamen eventus aerium PM-consociatum microbiommium, praesertim in ambitibus urbanis, late pervestigatum manet, [16] et — hoc tempore, nemo adhuc studia experimentalia speciatim destinavit. ad confirmandam vel excludendam praesentiam SARS-CoV-2 in PM.
Hic exhibemus primos eventus analysuum quas in 34 PM10 egimus exempla aerium PM10 e situ industriae Bergomensis Provinciae, collectis duobus diversis aerem samples per tempus continuam 3-ebdomadarum, a die 21 Februarii ad Martium. 13th.
Post methodum descriptam a Pan et al.anno 2019 (pro collectione, particula inspectione et deprehensione virus aerium), [17] PM exempla in vicus fibrarum filtrarum collecta sunt utendo samplera gravimetrici aeris low :2014 pro vigilantia PM10.Materia particularis comprehensa est in Filtra cum 99.9% typicaaerosol retentio, bene condita et tradita laboratorio applicatae et comparativo Genomics Universitatis Tergestinae.Dato "environmentali" specimen natura, nimirum in inhibitoribus polymerasium DNA locuples, cum RNA extractione processimus utendo solo Velox RNA fecali ornamentum microbeorum typo filtrorum accommodatum.[18]Dimidium colum volvebatur, summo latere ad interiora tendente;in 5 ml tubus polypropylene, una cum globulis in ornamentum.Ex initiali 1 ml lysisbuffer circiter 400 ul solutionis consequi potuimus, quae tum per protocolla norma definita processit, inde in finali 15 ul eluate.exim 5 ul adhibebantur ad SARS-CoV-2 tentantes.Datae praecipuae originis specimen, qScript XLT 1-Step RT-qPCR ToughMix adhibitum est.[19]Systema amplificationis protocolli a Corman et al, in QUI loco edito[20] sunt, evolvit.
Expertus expresse intendebatur ad confirmandam vel excludendam praesentiam SARS-CoV-2 RNA de re particulata.Prima analysis "E gene" pro hypothetica usus est et effectum positivum in 15 e 16 elaboravit infigo, etiamsi, ut exspectari possemus, cyclus erat inter 36-38 cyclos.
Post hoc analysin in 6 positivis filorum (iam positivi ad "E gene") replicavimus utendo "RtDR gene" tanquam hypotheticum titulum - quod valde specificum pro SARS-CoV-2 - attingens 5 eventus significantes of positio;potestate probationum ad excludendam positivity falsae etiam feliciter peractae sunt (fig. 1).
Ad evitandum currit extra materiam vix samplicationis in promptu, reliquae RNAs extractae traditae sunt Hospitali Universitatis locali (unum e centris clinicis ab auctoritate Italiae pro SARS-CoV-2 diagnostica probatis), ut alterum praestaret. parallela caeca test.Haec secunda laboratorium clinicum probatum 34 extractiones RNA pro E, N et RdRP genes, referentes 7 proventus positivum pro uno saltem e tribus genesis titulum, cum positivity distincte pro omnibus tribus figmentis confirmatis (Fig. 2).Propter specimen naturae, et considerans samplingum non ob orci diagnostica proposita, sed ad pollutiones environmental probationes exercendas (prae oculis habitis etiam columellae conditae sunt per quattuor saltem septimanas antequam hypotheticas geneticas analyses subiret, ut "Consecutio shutdown Italici), confirmare rationabiliter demonstrasse praesentiam SARS-CoV-2 viralem RNA, detecta valde specifica "RtDR gene" in 8 filtra.Attamen, ob defectum materiae additae e eliquationibus, satis numerum testium iterare non potuimus ut positivity omnibus 3 figmentis hypotheticis simul.
Hoc primum est praeiudicium quod SARS-CoV-2 RNA adesse potest in materia particulata velit, ita suggerens in condicionibus stabilitatis atmosphaerici et concentrationis AM, SARS-CoV-2, racemos velit PM et - per creare posse. reducendo diffusionem coefficientem – pertinaciam viri in atmosphaera augere.Praeterea confirmationes huius praeliminarisprobationes permanentes sunt, et aestimationem realem temporis comprehendere debet de vigore Sars-CoV-2 necnon eius rabie in materia particulari adsortata.Nunc, nullae suppositiones fieri possunt de ratione inter praesentiam viri in PM et COVID-19 tumultus progressum.Aliae quaestiones nominatim dirigendae sunt mediae concentrationes PM postearequiritur ad effectum potentialem contagionis (si id confirmatur quod PM ut "tabellarius" ageret pro nuclei viralis guttae), vel etiam theoretica possibilitas immunizationis consequentis minimi dosis ereptionis ad limina inferiorum AM. .
Fig.1 amplificatio curvarum E (A) et RdRP genes (B): lineae virides filtras probatas repraesentant;lineae cruciscolum extractiones referat referat;rubrae lineae amplificationem positivi exemplaria repraesentant.
Fig.2.Proventus positivus (cum X notatus) pro E, N et RdRP genes obtinuit pro omnibus specimen 34 PM10Filtra probata in secundis analysibus parallelis.
Leonardo Setti1, Fabricio Passarini2, Gianluigi De Gennaro3, Pierluigi Barbieri4, Maria Grazia Perrone5, Massimo Borelli6, Jolanda Palmisani3, Alessia Di Gilio3, Valentina Torboli6, Alberto Pallavicini6, Maurizio Ruscio7, Prisco Piscitelli8, Alessandro Miani8,9
1. Dept. Industriae Chemiae, Universitas Bononiensis, Viale del Risorgimento – 4, I-40136, Bologna, Italia
e-mail: leonardo.setti@unibo.it
2. Centrum interdepartmentale pro Industrial Investigatione "Fontes renovabiles, Environment, Blue Augmentum, Energy",
University of Bologna, Rimini, Italy e-mail: fabrizio.passarini@unibo.it
3. Dept. of Biology, University "Aldo Moro" of Bari, Bari, Italia
e-mail: gianluigi.degennaro@uniba.it; alessia.digilio@uniba.it; jolanda.palmisani@uniba.it
4. Dept.
e-mail: barbierp@units.it
5. Environmental Research Division, TCR TECORA, Milano, Italia
e-mail: mariagrazia.perrone@tcrtecora.com
6. Dept. of Life Sciences
e-mail: borelli@units.it; torboli@units.it; pallavic@units.it
7. Division Medicinae Laboratorium, Hospitalis Universitatis Iuliano Isontina (ASU GI), Tergesti, Italia
email: maurizio.ruscio@asugi.sanita.fvg.it
8. Italica Societas Environmental Medicine (SIMA), Milan, Italy
e-mail: priscofreedom@hotmail.com; alessandro.miani@unimi.it
9. Department of Scientiae Environmental and Poicy, University of Milan, Milan, Italy
e-mail: priscofreedom@hotmail.com; alessandro.miani@unimi.it
Corresponding author:
Leonardus Setti, Department of Industrial Chemistry, University of Bologna Viale del Risorgimento 4, 40136, Bologna, Italy; e-mail: leonardo.setti@unibo.it
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Deprehensio MMXIX coronavirus novellus (2019-nCoV) per real-time RT-PCR.Eurosurveillance, 25(3), available at:.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988269/
Original: https://doi.org/10.1101/2020.04.15.20065995
Post tempus: Apr-18-2020